Figure 1

Schematic representation of the flavonoid biosynthetic pathway in Arabidopsis thaliana seeds. Genes encoding enzymes for each step are indicated as follows: (1) Early biosynthesis genes: TRANSPARENT TESTA 4 (TT4), TRANSPARENT TESTA 5 (TT5), TRANSPARENT TESTA 6 (TT6) and TRANSPARENT TESTA 7 (TT7), which are involved in the biosynthesis of PAs precursors and other classes of Arabidopsis flavonoids. (2) Later biosynthesis genes (LBG): TRANSPARENT TESTA 3 (TT3), TRANSPARENT TESTA 18 (TT18) and BANYULS/ANTHOCYANIDIN REDUCTASE (BAN/ANR). 3) TRANSPARENT TESTA 12 (TT12, MATE transporter), TT10 (laccase 15), TT19 (Glutathione-S-transferase) and TRANSPARENT TESTA 13 (TT13). The regulatory control of LBGs requires the action of a MBW transcriptional regulation complex (MYB-bHLH-WDR), formed by a specific R2R3-MYB (TT2), bHLH transcription factors (EGL3, TT8) and a WD repeat protein TRANSPARENT TESTA GLABRA 1 (TTG1). Other transcription factors belonging to different families such as C2H2 and C2HC zinc finger (TT1/WIP1), MADS-box (ABS/TT16/AGL32, STK) and WRKY (TTG2/DSL1/WRKY44) also participate in their regulation.