Figure 3

Interploidy crosses in wildtype and tt8 using three accessions of Arabidopsis thaliana (Col, Ler and C24). (a) Mature F1 seeds obtained crossing diploid Col and tt8 mutant with diploid or tetraploid pollen from three Arabidopsis thaliana. (b) Percentage of plump F1 seeds from interploidy crosses using different Arabidopsis accessions as pollen donors to pollinate diploid Col and tt8 mutants. Data presented as means ± standard error. Around 200 F1 seeds were analyzed for each cross. Pooled data of three independent assays performed with 3 replicates for each measurement. (c) Seed size of F1 seeds resulting from interploidy crosses using three accessions of Arabidopsis pollen donors to pollinate diploid Col and 2 × tt8 (Col) mutants. Data is presented as means ± standard error. Pooled data of three independent assays performed with 3 replicates for each treatment (200–300 seeds/each). tt8 corresponds to tt8-6. One-way ANOVA followed by Tukey HSD test was used for analyzing significance. Crosses marked with the same lowercase letter display no statistical difference. Scales: 1 mm (a).