Figure 4

TT8 mRNA expression levels in seeds at 72 h after pollination from balanced and paternal excess crosses. tt8 corresponds to tt8-6. Data was normalized to ACTIN, with error bars indicating standard deviations based on four independent technical replicates. Statistical analysis was performed using Student’s t test (**p < 0.01). Three qPCR (biological replicates) were performed and generated similar results. Three independent biological replicates were performed.