Table 1 Genes and primer pairs of the endogenous reference gene used for RT- qPCR.
From: Screening and validating of endogenous reference genes in Chlorella sp. TLD 6B under abiotic stress
Gene symbol | Description | Accession ID | Primer sequence | Amplification size |
|---|---|---|---|---|
18S | 18S ribosomal RNA | Dech-6B_transcript_48114 | F:CTGGATCAGCGTTCGTCG | 170 |
R:TGATTTCAGCGGAGGTCAG | ||||
CYP | Cyclophilin | Dech-6B_transcript_14505 | F:TGGATGAGACGCACCTAGTTGTG | 110 |
R:CTGGAAGAAGGGGCTGTTGG | ||||
EF1α | Elongation factor 1α | Dech-6B_transcript_9145 | F:GATGGCTGAGGTGGTGTTTGAGC | 145 |
R:TTACGCCTTGGGGGTGGTCT | ||||
GAPDH | Glyceraldehyde-3-phosphate Dehydrogenase | Dech-6B_transcript_44570 | F:CCCTCTCTTGCCCTTTCTCCGT | 156 |
R:GGTCTTGGGTTGTGCTTGCTGC | ||||
GTP | GTP binding protein | Dech-6B_transcript_17752 | F:TTTCCGCCCGCTTGGAAT | 118 |
R:GCGTGATGATGGAGGTTTTGC | ||||
IDH | NADP-isocitrate dehydrogenase | Dech-6B_transcript_39928 | F:CTGGAAGAAGCCGATTGTGGTG | 154 |
R:GCCCTTGAAGTCGTAGATGGTGAA | ||||
UBC | Ubiquitin-conjugating enzyme | Dech-6B_transcript_34066 | F:CGGGAGATGCCGACTACTGC | 107 |
R:CAGCCCTGTCCTGGTTTTCTAA | ||||
α-TUB | Tubulin alpha chain | Dech-6B_transcript_45761 | F:CGGCTGCTTGAGGCGTCGTT | 111 |
R:GCTTGCCCAATGTGGATAGAAATAA | ||||
β-TUB | Tubulin beta chain | Dech-6B_transcript_29059 | F:GCAACAACTGGGCCAAGGG | 198 |
R:TCATGCGGTCGGGGTACTCC | ||||
PXG3 | Peroxygenase 3 | Dech-6B_transcript_13983 | F:AGCGACAGCGAGATCTT | 146 |
R:CAACCCAGCCGACAAAG | ||||
POD | Peroxidase | Dech-6B_transcript_57541 | F:CGCAGTTTGTTGCACTGC | 107 |
R:CCACCATCTCCTGGTCGT |
