Figure 2

Bd inhibition curves of hellbender skin peptides grouped by region. Line graphs show inhibition curves with standard error bars of % Bd zoospore growth based on positive controls against increasing concentrations of skin peptides collected from both hellbender subspecies: Ozark hellbenders are from Arkansas (AR; red), and eastern hellbenders from Middle Tennessee (MTN; yellow), and East TN (ETN; teal). Anti-Bd activity of peptides from all individuals were tested up to 1000 µg/mL (n = 39; 5 AR, 10 MTN, 24 ETN) with a subset tested out to 2500 µg/mL (n = 16; 5 AR, 5 MTN, 6 ETN).