Figure 3

Differential inhibition of two chytrid species. Batrachochytrium dendrobatidis (Bd; green) and B. salamandrivorans (Bsal; purple), show different inhibition profiles when challenged against varying hellbender skin peptides concentrations. Left shows curves with standard error bars of % zoospore growth in comparison to positive controls against increasing peptide concentrations of 25 to 2500 µg/mL. Right shows bar graphs with standard error bars representing % zoospore growth at 500 µg/mL peptide concentrations, separated by subspecies of Ozark hellbenders of Arkansas and eastern hellbenders of Middle and East Tennessee. Growth inhibition assays for Bd and Bsal comparisons were performed from the same peptide samples (n = 21, 4 Ozark, 17 eastern).