Figure 4
MALDI-TOF profiles from 1000 to 4000 Da of four skin peptide samples collected passively from hellbenders. Comparison can be seen between the most inhibitory samples of Batrachochytrium dendrobatids at 500 µg/mL peptide concentration (Bd zoospore growth < 10%; Category 5), and the least inhibitory samples (Bd zoospore growth > 90%; Category 1). Top two profile are from individuals of eastern hellbenders from East Tennessee (ETN) that exhibited complete inhibition of Bd zoospores (Category 1). The bottom two profiles include an eastern hellbender from ETN and an Ozark hellbender from AR that exhibited very little Bd zoospore inhibition (Category 5). While profiles showed considerable variation, all samples evaluated included strong peaks around m/z 1547. Note also that the two samples of Category 5 have peaks of around m/z 1122 as well as either peaks around m/z 2879 or m/z 2896; these three peptide masses were considered indicative of strong inhibition based on indicator species analysis.
