Figure 1
From: A proteome scale study reveals how plastic surfaces and agitation promote protein aggregation
(A) Scheme of the experimental setup designed to measure protein loss during agitation of purified protein solutions with a rotating wheel. The speed ranged from 0 to 3 rpm, corresponding to a power from 0 to 0.12 W/kg. Tubes made of PP, glass, TEFLON, LOBIND were used. A conical PP FISHERBRAND tube is represented. (B) Protein loss of BSA, Hb, α-syn solutions measured in PP, glass, TEFLON and LOBIND tubes after 24 h at 6 °C without (grey) and with agitation at 3 rpm (green) on a rotating wheel. The initial protein concentration was C0 = 0.1 g/L (V = 10 mL). Significant differences are determined using the Tukey’s “honestly significant difference” test realized from variance analysis (Anova) (*** p-value < 0.001; ** p-value < 0.01). To observe the effect of the protein nature, only the differences at 3 rpm are shown, all the statistical results are available in Table S3.
