Table 2 Comparison of protein binding results from in vitro and in vivo methods.

From: Developmental changes in the extent of drug binding to rat plasma proteins

Drug

In vitro (%)

In vivo (%)

Valproate

92.1 ± 1.3*

78.2 ± 7.7*

Lamotrigine

53.8 ± 4.2

60.2 ± 3.1

  1. Protein-free fraction/whole plasma concentration ratios (%) in plasma of P4 rats from experiments conducted either in vitro (spiked with valproate or lamotrigine with respective [3H]-labelled drugs) or in vivo (plasma collected from animals injected with unlabelled drugs estimated by LC–MS/MS). Mean ± SD; n = 3–4. *p < 0.05.
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