Figure 2
From: Direct cell extraction of membrane proteins for structure–function analysis
Salipro-mPANX1 structure and functional analysis. (A) Schematic of His-Salipro-mPANX1 nanoparticles immobilisation to a sensor with Ni2+ ions (NiHC) binding to the His-tag. Concentration series of bzATP (B) and spironolactone (C) was injected over a His-Salipro-mPANX1 coated surface with a calculated KD of 720 μM ± 133 μM and 160 μM ± 10 μM, respectively. (D) Cryo-EM reconstruction of Salipro-mPANX1 at 3.1 Å. The ion channel (orange) and Salipro lipid disk (blue) is shown as side view (left) and top view (right). (E) The structural model of mPANX1 with each subunit colored. All SPR data is representative of n = 3.
