Figure 2

Combined stressors and biological sex differences in spatial learning. Radial Arm Water Maze (RAWM) was used to measure spatial learning under different housing and treatment conditions. Total Average Errors over 6 trials for Males (a) and Females (b). A two-way ANOVA showed a significant interaction between GCR and Housing for Total Average Errors (F(2,54) = 4.838, p = 0.012, η² = 0.152). The simple main effect of GCR (F(1,54) = 8.340, p = 0.006, η² = 0.134) showed a significant increase in average errors in 50 cGy compared to 0 cGy group housed males (mean diff 1.233, 95%CI(.377 to 2.090), p = 0.006), and the simple main effect of housing (F(2,54) = 6.036, p = 0.004, η² = 0.183) showed a significant increase in average errors in 0 cGy SI males compared to 0 cGy group housed (mean diff 1.293, 95%CI(.238 to 2.349), p = 0.011) and SI + HU housed males (mean diff 1.277, 95%CI (.221 to 2.332), p = 0.013). Males: n = 10 per group for 60 total mice. Females: n = 10 for all groups except Group 50 cGy (n = 9), SI 0 cGy (n = 8) for 57 total mice. *p < 0.05, **p < 0.01.