Figure 5
Activation of both Gαi/o and Gαq/11 is important for synergistic calcium mobilization induced by costimulation of CXCR4 and HRH1. MDA-MB-231 cells were pretreated with vehicle (A), PTX (100 ng/ml, overnight) (B), or YM254890 (300 nM, 30 min) (C) prior to agonist treatment. Cells were stimulated with 3 nM CXCL12, 100 nM histamine, or both, and intracellular calcium flux was measured. (D) The area under the curve of each calcium response was calculated and normalized to that of CXCL12. Data are expressed as mean ± s.e.m. (n = 3). ***P < 0.001.
