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Figure 2

From: Engineering of Chinese hamster ovary cells for co-overexpressing MYC and XBP1s increased cell proliferation and recombinant EPO production

Figure 2

Development of a MYC and XBP1s overexpressing CHO cell line. (black) CHOEPO cell line; (red) CHOCEPO cell line; (green) CHOXEPO cell line; (blue) CHOCXEPO cell line. (A) Overview of the cell engineering strategy to overexpress MYC and XBP1s in the CHOEPO cell line. The CHOXCEPO cell line was generated by transfecting cells with the CMV-MYC-PuroR and the CMV-XBP1s-HygroR plasmid, and isolating cells resistant to puromycin and hygromycin in the culture medium. Additionally, the CHOCEPO and CHOXEPO cell lines were generated as control cell lines. (B) Integral of viable cell concentration (IVCC). (C) cell-specific growth rate (µ). (D) EPO titre. (E) cell-specific EPO productivity (qEPO). (F) Glucose consumption rate (qGLC). (G) Lactate production rate (qLAC). (H) Ratio of lactate produced to glucose consumed (YLAC/GLC). (I) Glutamine consumption rate (qGLN). (J) Ammonia production rate (qNH3). (K) Ratio of ammonia produced to glutamine consumed (YNH3/GLN). Each dot corresponds to the duplicates (n = 2) of the three isolated cell clones. p values (One-way ANOVA) below to 0.05 indicate the statistical significance of parameter variation.

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