Figure 3 | Scientific Reports

Figure 3

From: Engineering of Chinese hamster ovary cells for co-overexpressing MYC and XBP1s increased cell proliferation and recombinant EPO production

Figure 3

Relative mRNA expression of recombinant EPO, MYC and XBP1 and their relationship with recombinant EPO production. (black) CHOEPO cell line; (red) CHOCEPO cell line; (green) CHOXEPO cell line; (blue) CHOCXEPO cell line. (A,B) and C) mRNA expression of EPO, MYC and XBP1, respectively, normalised using GAPDH as internal standard. Samples for mRNA expression analysis correspond to day 3. (D–F) Comparison between normalised EPO titres (x-axis) and mRNA levels of EPO, MYC and XBP1s (y-axis), respectively. (G–I) Comparison between normalised IVCC titres (x-axis) and mRNA levels of EPO, MYC and XBP1s (y-axis), respectively. Each dot corresponds to a replicate derived from CHOEPO cells as well as from three independent single-cell derived CHOCEPO , CHOXEPO and CHOCXEPO cell lines.

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