Figure 4 | Scientific Reports

Figure 4

From: Engineering of Chinese hamster ovary cells for co-overexpressing MYC and XBP1s increased cell proliferation and recombinant EPO production

Figure 4

Comparison of glucose and temperature effect on the culture performance of CHOEPO and CHOCXEPO cell lines. (black) CHOEPO in 20 mM glucose, (grey) CHOEPO in 40 mM glucose, (dark blue) CHOCXEPO in 20 mM glucose; (light blue) CHOCXEPO in 40 mM glucose. (A) Overview of the culture conditions evaluated for both cell lines. (B) Integral of viable cell concentration. (C) Cell-specific growth rate. (D) EPO titre. (E) Cell-specific productivity. Experimental values represent the mean of two biological replicates ± SEM. (*), (**) and (***) indicated p < 0.05, 0.001, 0.0001 (Two-way ANOVA). Culture of CHOEPO at 37 °C and 20 mM glucose was the control condition.

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