Figure 2
Interferometric deformability cytometry properties of immature BMDCs. (A) Cell length calculated as cell time × velocity, (B) maximum, (C) time ratio and (D) mid-slope shift, represented at varying cell velocities (x axis, m/s). Horizontal dashed lines are the respective parameter values of a centered nucleus with a time ratio at 1 and a mid-slope shift at 0. Data was acquired in three independent experiments, where each experiment tested different velocities. Experiment 1 acquired data for the three lowest cell velocities, experiment 2 for the five subsequent cell velocities and experiment 3 for the 5 highest cell velocities. Each cell velocity was acquired on various numbers of cells ranging from 13,292 to 28,437 cells, with an average of ~ 22,000 cells per velocity. For each velocity, a box plot is used to represent the measured parameter population. The box plots show the median value, upper and lower quartile (box), maximum and minimum values for each sample.
