Figure 5
From: RNA disruption is a widespread phenomenon associated with stress-induced cell death in tumour cells
Effect of doxorubicin on cell viability/death and RNA integrity in three non-tumourigenic cell lines. Human umbilical vein endothelial cells (HUVECs) (left panels), mouse NIH3T3 fibroblast cells (middle panels) and human MCF-10A breast epithelial cells (right panels) were treated with doxorubicin for 72 h. (a) RNA disruption assay. Total RNA was isolated from cells, and RNA disruption was quantified using the RDA. (b) Cell counting assay. Total cells were counted using a haemocytometer following drug treatment. Untreated cells were counted prior to treatment (‘0 h’) to provide a baseline count. (c) DNA content analysis. Drug-treated cells were collected, washed, fixed and stained with propidium iodide then analysed by flow cytometry. Data are presented as means ± standard deviation, with individual data points shown in red. Groups labelled with an asterisk were significantly greater (panels a and c) or significantly lower (panel b) than the ‘0 h’ (panel b) or untreated (panels a and c) control. See Supplementary Table S4 for detailed results of each statistical test.
