Figure 4
From: An mTOR feedback loop mediates the ‘flare’ (‘rebound’) response to MET tyrosine kinase inhibition
Phosphorylation on S50 and S378 inhibits the activity of PTP1B. (A) EBC1 and HS746T cells were treated with DMSO or JNJ-605. After WO, cells were transiently transfected with indicated vectors (JNJ + WO condition corresponds to transfection with an empty vector). All vectors containing the cDNA of PTP1B were N-terminally tagged with HA. Cellular lysates were immunoblotted, as indicated on the left. Black-edged rectangles delineate the blot. (B) Cells were treated and transfected as in (A) and subjected to viability assay 72 h post-WO and post-transfection. Results are the mean fold change over JNJ-605-treated cells without WO +/− SEM with N = 6. ANOVA was performed to test statistical significance with Tukey’s post-hoc test with ns: not significant; *P < 0.05; **P < 0.01; ***P < 1.10–3; ****P < 1.10–4.
