Figure 3
Seed and leaf glucosinolate contents in populations segregating for BnMYB28 and BnCYP79F1 mutations. F2 populations 200527 and 200529 segregating for BnMYB28 and BnCYP79F1 mutations, respectively, originated from M3xM3 crosses. Homozygous F2 double mutants (A1A1B1B1 and C1C1D1D1) were analyzed together with homozygous single mutants (A1A1BeBe, AeAeB1B1, C1C1DeDe, and CeCeD1D1), non-mutagenized Express617 and F2 plants homozygous for the wildtype alleles (AeAeBeBe and CeCeDeDe). Leaf samples were taken 15 days after pollination and mature seeds (BBCH89) were used for glucosinolate determination. Error bars represent the standard error from five plants (n = 5) per genotype with two exceptions mentioned in the figure. An ANOVA (p < 0.05) was performed and the Tukey test (p < 0.05) was done for grouping. Different alphabets above error bars represent groups based on significance. All genotypes are as per designated allele codes given in Table 2.
