Figure 4
Analysis of major glucosinolate types in mature seeds of BnMYB28 and BnCYP79F1 mutants. Aliphatic and other GSL types measured from homozygous BnMYB28 (genotype A1A1B1B1, seed code 200527) and BnCYP79F1 (genotype C1C1D1D1, seed code 200529) originating from M3xM3 crosses. F2 plants homozygous for the wildtype alleles (AeAeBeBe and CeCeDeDe) and non-mutated Express617 were used as controls. Plants were grown in the greenhouse. Mature seeds were harvested at BBCH89. GSL profiles for aliphatic and other GSL types (phenolic and indolic) were analyzed using HPLC. GSL content was calculated as µmol/g dry weight (DW). Individual and mean values are marked in black and red dots, respectively. Blue triangles represent outliers. Error bars represent the standard error of the mean from five biological replicates. An ANOVA test (p < 0.05) was performed and significant differences between groups were calculated by a Tukey test (p < 0.05). Different alphabets above error bars represent groups based on significance. All genotypes are as per designated allele codes given in Table 2.
