Figure 5
Analysis of aliphatic glucosinolates in mature seeds of BnMYB28 and BnCYP79F1 mutants. Individual aliphatic GSLs in homozygous F2 BnMYB28 (genotype A1A1B1B1, seed code 200527) and BnCYP79F1 (genotype C1C1D1D1, seed code 200529) double mutants originating from M3xM3 crosses. F2 plants homozygous for the wildtype alleles (AeAeBeBe and CeCeDeDe) and non-mutated Express617 were used as controls. Aliphatic GSLs were identified and quantified using HPLC. The content was calculated as µmol/g dry weight (DW). Individual and mean values are marked in black and red dots, respectively. Blue triangles represent outliers. Error bars represent the standard error of the mean from biological replicates. An ANOVA test (p < 0.05) was performed and significant differences between groups were calculated by a Tukey test (p < 0.05). Different alphabets above error bars represent groups based on significance. All genotypes are as per designated allele codes given in Table 2. n.d not detectable.
