Table 2 Allele codes assigned to EMS mutants and wildtype plants selected as crossing parents in this study.

From: Reduced glucosinolate content in oilseed rape (Brassica napus L.) by random mutagenesis of BnMYB28 and BnCYP79F1 genes

 

Gene name

Mutation position on gDNA1

Allele code

cDNA change1

AA change

Mutation type

M3 seed code2

M3 single mutants

BnMYB28.C09

G51A

A1

G51A

W17*

Nonsense

190623

BnMYB28.A03

G50A

B1

G50A

W17*

Nonsense

190625

BnCYP79F1.C05

C424T

C1

C424T

E142*

Nonsense

190628

BnCYP79F1.A06

G1379A

D1

G1090A

E364K

Missense

190630

Wildtype Express617

BnMYB28.C09

n.a

Ae

n.a

BnMYB28.A03

Be

BnCYP79F1.C05

Ce

BnCYP79F1.A06

De

  1. Single mutants were selected as crossing parents to combine single mutations for enhanced phenotypic effects. For each of the analyzed paralogs, mutants and wildtype parents were assigned unique allele codes.
  2. 1Position relative to the translation start site.
  3. 2M3 seed codes corresponding to screened M2 mutants.
  4. Non-mutated wildtype alleles from Express617 are represented with the ‘e’ suffix in subscript.
  5. *Premature stop codon, n.a not applicable.
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