Figure 4

Representative Fluorescent confocal images (successive slices) showing the distribution of PET-NR nanoparticles in the body of zebrafish embryos (96 hpf) that were exposed to 100 ppm PET-NR nanoparticles for 24 h as compared to embryos treated with only NR (control). As can be noticed, nanoparticles are accumulated at various locations including intestine (a), pronephric duct/kidney (b), liver (c) and brain. Images were acquired using inverted laser-scanning confocal microscope (Leica DMi8 / TL LED, Leica Microsystems CMS GmbH). Scale bar: 1 mm.