Figure 7 | Scientific Reports

Figure 7

From: A mechanistic understanding of the effects of polyethylene terephthalate nanoplastics in the zebrafish (Danio rerio) embryo

Figure 7

(A) Localization of reactive oxygen species (ROS) production in zebrafish embryos exposed to PET NPs (100 ppm) as compared to control embryos. Embryos (96 hpf which were already treated with PET for 24 h) were incubated for 60 min in CM-H2DCFA (10 μM) in rearing medium. Fluorescence detected in different regions of PET NPs treated embryos including (a) Intestine), (b) gall bladder and (c) liver. Scale bar: 1 mm. (B) Glutathione levels in extracts of zebrafish embryos (72 hpf) exposed to PET NPs (100 ppm for 24 h) as compared to control (Cont) embryos. Glutathione (GSH) levels were analysed by using GSH assay kit from Sigma-Aldrich. Significant reduction of GSH (*p < 0.05; n = 6) in PET NPs-treated embryo is clearly observed.

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