Figure 1 | Scientific Reports

Figure 1

From: Simultaneous detection of omicron and other SARS-CoV-2 variants by multiplex PCR MassARRAY technology

Figure 1

The molecular principle of the MassARRAY method. The analysis begins with reverse transcription of viral RNA into cDNA and amplification by multiplex PCR in a single reaction. Next, PCR products are treated with Shrimp Alkaline Phosphatase (SAP) enzyme that removes unincorporated nucleotides, and a multiplex primer extension (MPE) reaction is carried out. In the MPE reaction, each product is detected at a specific mass because of the addition of terminating nucleotides, which are complementary to the template sequence.

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