Figure 4
Histological analysis of the non-decalcified specimens. (A) Van Gieson's staining of the specimens in each group. The new bone was stained into red. The periosteum-covered group showed only limited residual DBBM encapsulated by fibrous tissue in the grafted area and higher new bone area than the other two groups. A small amount of new bone with much more DBBM remnants was observed in the Bio-Gide membrane-covered group, (B) Percentage of new bone area and residual DBBM area were assessed by histomorphometric measurement. The periosteum group showed significant higher new bone area and lower residual DBMM area than the Bio-Gide group, which demonstrated that the periosteum evidently accelerated the formation of new bone (*P < 0.05; **P < 0.01).
