Figure 3
From: LAMP3 transfer via extracellular particles induces apoptosis in Sjögren’s disease
A fraction of extracellular particles from LAMP3-overexpressing A253 cells induces apoptosis in naïve A253 cells. (A) Schematic methods. Two fractions of extracellular particles (EPs) were separated from culture medium of control, LAMP3-overexpressing, or LAMP1-overexpressing A253 cells using centrifugation in combination with or without total exosome isolation reagent. Naïve A253 cells were treated with (B) EP fraction 2, (C) EP fraction 1 or (D) heat-denatured (heat-treated) EP fraction 1. Apoptotic cells in naïve cell culture were determined by flow cytometry using APC Annexin V/7-AAD 72 h after incubation. Graphs show difference in mean (± SD) number of Annexin V+/7-AAD+ cells in cell culture compared with control (n = 3 for all the experiments). **p < 0.01 (one-way ANOVA).
