Figure 6
From: LAMP3 transfer via extracellular particles induces apoptosis in Sjögren’s disease
Transfection of recombinant LAMP3 protein induces apoptosis in A253 cells. (A) Representative Western blots of A253 cells transfected with recombinant LAMP3 (rLAMP3), recombinant LAMP1 (rLAMP1), or control FLAG peptide (uncropped images are provided in Supplementary Fig. 3). (B) Apoptotic cells were determined in A253 cell culture by flow cytometry using APC Annexin V/7-AAD 14 h after transfection. Graph showing difference in mean (± SD) number of Annexin V+/7-AAD+ cells in A253 cell culture transfected with rLAMP3 or rLAMP1 compared with control. (C) A253 cells were transfected with rLAMP3, heat-treated rLAMP3, or FLAG peptide. Apoptotic cells were determined in A253 cell culture by flow cytometry using APC Annexin V/7-AAD 14 h after transfection. Graph showing difference in mean (± SD) number of Annexin V+/7-AAD+ cells in A253 cell culture transfected with rLAMP3 or heat-treated rLAMP3 compared with control. (D) A253 cells were treated with control FLAG peptide or rLAMP3 alone (without transfection reagent) or transfected with control FLAG peptide or rLAMP3 with transection reagent. After 14 h, number of apoptotic cells was determined by flow cytometry using APC Annexin V/7-AAD. Graph showing difference in mean (± SD) number of Annexin V + /7-AAD + cells in cell culture treated with rLAMP3 alone or transfected with FLAG peptide or rLAMP3 compared with FLAG peptide alone. **p < 0.01 (one-way ANOVA, n = 4 for all the experiments).
