Figure 6
A photomicrograph of kidney cortex sections of rats showing; (a–d) Masson’s trichrome stain: (a) Control rats showing fine collagenous fibres surrounding Bowman's capsule (↑) and the blood vessels (↑↑). (b) BPA-treated rats showing large amounts of collagenous fibres around the dilated, thickened wall of the blood vessel (bv), between the renal tubules (↑) and around Bowman’s capsule (head arrow). (c) BPA + G50-treated rats showing a moderate amount of collagenous fibres around the Bowman’s capsule, between the renal tubules, and around renal blood vessels. (d) BPA + G200-treated rats had less collagenous fibres around the Bowman’s capsule, between the cortical tubules, and around the blood. (e–h) PAS reaction: (e) Control rats showing a strong PAS + ve reaction of polysaccharides in glomerular tufts of capillaries (G), Bowman’s capsule (↑), brush borders (head arrow), and the basement membrane of renal tubules (↑↑). (f) BPA-treated rats showing a decrease in the carbohydrate content of many renal tubules (*) and Malpighian corpuscles. (g) BPA + G50-treated rats showing positive PAS reactions in many renal tubules (↑↑), Bowman’s capsule (↑) and glomerular tufts of capillaries (G). (h) BPA + G200-treated rats exhibited strong PAS + ve reactions in the majority of renal tubules, Bowman’s capsule, and glomerular capillary tufts (× 40).
