Figure 5 | Scientific Reports

Figure 5

From: Engineering of an electrically charged hydrogel implanted into a traumatic brain injury model for stepwise neuronal tissue reconstruction

Figure 5

In vitro three-dimensional cultures of NSCs on the C1A1 porous hydrogel. (a) Differential interference contrast (DIC) image of NSCs 2 weeks after seeding into the C1A1 porous hydrogel. bFGF (10 ng/ml) was added daily for the first week, and bFGF was removed for the second week. The inset is a higher magnification of the area in the red square. (b) Immunofluorescence image. Three-dimensional cultures of neurons and glial cells were confirmed to stain red for βIII tubulin and green for myelin basic protein. Blue indicates nuclei visualized by DAPI staining. (c) Immunofluorescence images of areas of confirmed myelination. The white box indicated in the left panel is magnified and shown as the middle panel. The crossing point of the yellow vertical and horizontal lines is magnified and shown as lower and right panels. White bars indicate 50 μm and 20 μm in the left and middle panels, respectively.

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