Figure 1

Repeatability of AOFLIO and phasor in partitioning the same cones. Representative fluorescence lifetime data acquired with a low-level exposure (3 mW, 877 J/cm2) taken on two different days at the same location (i.e., initial exposure (a–c) and Paradigm 1 (d–f)). In each fluorescence intensity image (a,d), the same 348 cones were marked (b,e) in red or blue according to their GMM cluster assignments in the phasor plots (c,f). Each phasor coordinate represents one cone. In both exposures, the same 28 cones, representing 8.1% of all cones, were assigned to Cluster 1. Cones obscured by the blood vessel shadow in the right side of the image were not marked. Scale bar represents 20 μm.