Table 5 Prediction and validation of optimized formulation (DIP).
Factor | Coded value | Actual value (μM) | Response | Predicted value | Experimental valuea | % Error |
|---|---|---|---|---|---|---|
Span 60 | 0.728 | 13.98 | Zeta potential (mV) | − 28.054 | − 28.49 ± 1.01 | 0.037 |
Cholesterol | − 0.303 | 6.697 | Vesicle size (nm) | 202.582 | 195.36 ± 4.22 | 3.24 |
Egg lecithin | − 0.882 | 6.354 | Entrapment efficiency of Dox (%) | 78.121 | 77.24 ± 1.45 | 0.198 |
|  |  |  | Entrapment Efficiency of ICG (%) | 90.174 | 91.21 ± 0.91 | 0.099 |
|  |  |  | Invitro drug release at 72nd hour (%) | 42.994 | 40.71 ± 3.21 | 0.438 |
|  |  |  | NIR Hyperthermia temperature (°C) | 54.636 | 56.17 ± 0.94 | 0.165 |
