Figure 2 | Scientific Reports

Figure 2

From: Laboratory protocol is important to improve the correlation between target copies and metabarcoding read numbers of seed DNA in ground beetle regurgitates

Figure 2

Correlation of copies/µl and read numbers of the plant-specific trnL c-h target fragment in regurgitates of Pseudoophonus rufipes collected at 0, 4, 8, 16, 24, 32, 48, 64, 72 and 96 h after feeding on 1–3 seeds of T. officinale. Dots in the scatterplot (○) are measured values, black bold lines are predicted values of a linear regression model of copies and reads, dashed lines are 95% confidence intervals, shaded area marks boot-strapped 95% confidence interval, and R2 indicates goodness of model-fit.

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