Figure 4 | Scientific Reports

Figure 4

From: Laboratory protocol is important to improve the correlation between target copies and metabarcoding read numbers of seed DNA in ground beetle regurgitates

Figure 4

Correlations of plant-specific trnL-target copies/µl DNA extract and signal strength (RFU) of celPCRs products after 25, 30, 35 or 40 cycles with a reaction volume of 10 µl. Dots (○) are measured values, black bold lines are predicted values of linear regression models, dashed lines are 95% confidence intervals, shaded area are boot-strapped 95% confidence intervals and R2 indicates goodness of model-fit.

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