Figure 2
From: Non-invasive screening of breast cancer from fingertip smears—a proof of concept study
Column graph showing the number, the absolute and relative intensities of either all peptides peaks (a–c, respectively) or potential breast cancer biomarkers (d–f, respectively) present in fingermarks spotted with several digestion conditions (n = 3): (i) 25 µg/mL trypsin containing 0.01% v/v glycerol and incubated in Tupperware box containing saturated K2SO4 solution at 50 \(^\circ\)C for 2 h; panel (ii): fingermark spotted using set (i) conditions except for trypsin used at a concentration of 20 μg/mL and RapiGestSF at 0.1% replacing glycerol; (iii) fingermark spotted using 20 μg/mL trypsin containing 0.1% RapiGestSF and incubated at 37 \(^\circ\)C for 3 h in a Tupperware box containing water wet paper; (iv): fingermark spotted using same conditions as set (iii) but replacing the wet paper with 50:50 H2O:MeOH solution. For relative intensities, these were normalised with the autolysis peptide peak of trypsin at m/z 842.5094.
