Figure 4

Column graph showing the number, the absolute and relative intensities of either all detected peptides peaks (a,b, c, respectively), or of potential breast cancer biomarkers (d,e,f, respectively) in fingermarks incubated for 2 h at 50 °C in Tupperware box, containing a saturated solution of K₂SO₄, after spotting them with a 20 μg/mL trypsin solution in 50 mM NH₄HCO₃ buffer containing either Set (vi) -RapiGest^SF 0.1% (w/v); Set (ii)—RapiGest^SF 0.2% (w/v); Set (iii)—MEGA-8 2% (w/v); Set (iv)—a mixture of 2% (w/v) MEGA-8 and 0.1% (w/v) RapiGest^SF. For relative intensities calculation, peptides intensities were normalised against the autolysis fragment of trypsin at m/z 842.509.