Table 2 Putative protein identification from in situ digests of ungroomed fingermarks spotted with set (i): 25 µg/mL trypsin containing 0.01% v/v glycerol and incubated in a Tupperware box containing saturated K2SO4 solution at 50 °C for 2 h; set (ii): set (i) conditions except for trypsin used at a concentration of 20 μg/mL and RapiGestSF at 0.1% replacing glycerol; set (iii) 20 μg/mL trypsin containing 0.1% RapiGestSF and incubated at 37 °C for 3 h in Tupperware box containing water-wet paper; set (iv): same conditions as set (iii) but replacing the wet paper with 50:50 H2O:MeOH solution. The asterisk indicates the proteins that are reported as breast cancer biomarkers in the literature.
From: Non-invasive screening of breast cancer from fingertip smears—a proof of concept study
Proteolytic conditions | Protein | Peptide m/z | Sequence | Mass accuracy (ppm) |
|---|---|---|---|---|
Set (i) (use of K2S04) | *Dermcidin | 1128.523 | ENAGEDPGLAR | −4.7 |
Keratin, type I cytoskeletal 9 | 1065.509 1157.588 1791.724 | STMQELNSR QGVDADINGLR GGSGGSYGGGGSGGGYGGGSGSR | 9.1 −2.1 −1.8 | |
Filaggrin | 1421.638 | SESASRNHYGSAR | −9.4 | |
Sortilin-related receptor | 1232.595 | IEVANPDGDFR | 3.6 | |
*Calmodulin like protein 3 | 1874.849 | EAFSLFDKDGDGCITTR | −5.2 | |
Set (ii) (use of K2S04) | *Dermcidin | 725.392 1128.524 | GAVHDVK ENAGEDPGLAR | −2.6 −3.3 |
Keratin, type II cytoskeletal 1 | 1033.511 1277.708 | TLLEGEESR LALDLEIATYR | −4.7 −1.6 | |
Keratin, type I cytoskeletal 9 | 1157.590 1066.506 | QGVDADINGLR FEMEQNLR | −0.5 7.1 | |
Filaggrin | 1493.731 1513.728 | QGSHHKQARDSSR QGSRHEQARDSSR | −0.5 4.3 | |
Desmoplakin | 1107.536 | AEFQEEAKR | −5.9 | |
Protein Shroom 3 | 1201.604 | SLADILDPDSR | −1.9 | |
*Zinc a2 glycoprotein | 1475.757 | WEAEPVYVQRAK | −4.8 | |
Set (iii) (use of wet paper) | *Dermcidin | 1128.522 | ENAGEDPGLAR | −5.0 |
Filaggrin | 1513.728 | QGSRHEQARDSSR | 4.4 | |
Keratin, type II cytoskeletal 1 | 1308.655 | NKYEDEINKR | 0.8 | |
Desmoplakin | 1254.594 1818.990 | AITGFDDPFSGK IDKQIDFRLWDLEK | −5.2 8.2 | |
Filamin B | 1373.651 | WCNEHLKCVNK | 4.1 | |
Keratin, type I cytoskeletal10 | 1996.969 | ELTTEIDNNIEQISSYK | −0.9 | |
Myosin-6 | 718.398 | KGFPNR | −1.5 | |
Set (iv) (use of 50:50 H20/MeOH) | *Dermcidin | 1128.530 | ENAGEDPGLAR | 2.0 |
Keratin, type I cytoskeletal 9 | 1791.737 982.436 | GGSGGSYGGGGSGGGYGGGSGSR FSSSGGGGGGGR | 5.5 2.7 | |
Desmoplakin | 1254.593 | AITGFDDPFSGK | −5.6 | |
Keratin, type II cytoskeletal 1 | 1006.431 832.492 | GGSGGGGGGSSGGR SISISVAR | 1.5 3.5 |
