Table 3 Comparisons of differentially expressed phosphorylated peptides related to metabolic pathways treated with PZH and sorafenib from TMT phosphoproteomics and PRM verification.

Uniprot	KEGG pathways	Protein description Peptide name	Phosphorylation fold change
			PZH versus control		Sora versus control		PZH versus sora
			TMT Phospho proteomics	PRM	TMT Phospho proteomics	PRM	TMT Phospho proteomics	PRM
O70250	Glucagon signaling pathway Glycolysis/gluconeogenesis	Phosphoglycerate mutase 2 (PGAM2)	\	\	\	\	\	\
		Peptide (residues 129–139): HGESLWNQENR	0.68#	0.63**	0.88*	0.79*	0.64**	0.80#
		Peptide (residues 12–20): HNYYTSISK	0.48*	0.41**	1.21	0.64**	0.55**	0.63*
P06151		L-lactate dehydrogenase A chain (LDHA)	\	\	\	\	\	\
P06151		Peptide (residues 233–243): QVVDSAYEVIK	0.66	0.45**	1.23	0.72*	0.53**	0.62**
P47857	Glycolysis/gluconeogenesis HIF-1 signaling pathway Fructose and mannose metabolism	ATP dependent 6-phosphofructokinase (PFK1)	\	\	\	\	\	\
P47857		Peptide (residues 657–673): NVLGHMQQGGSPTPFDR	0.89	0.25**	1.12	0.23**	0.79*	1.09
P05064		Fructose bisphosphate aldolase A (ALDOA)	\	\	\	\	\	\
		Peptide (residues 44–56): LQSIGTENTEENR	0.84	0.34**	1.18*	0.54*	0.71**	0.53*
		Peptide (residues 29–42): GILAADESTGSIAK	0.57	0.49**	1.50*	0.64**	0.45**	0.76*
Q9QXG4	Glycolysis/gluconeogenesis Pyruvate, propanoate metabolism	Acetyl-coenzyme A synthetase (ACSS2)	\	\	\	\	\	\
Q9QXG4	Glycolysis/gluconeogenesis Pyruvate, propanoate metabolism	Peptide (residues 28–36): GWSPPPEVR	0.89	1.08	1.32**	1.26*	0.54*	0.86
P41216	PPAR signaling pathway Fatty acid biosynthesis/degradation	Long chain fatty acid CoA ligase (ACSL1)	\	\	\	\	\	\
P41216	PPAR signaling pathway Fatty acid biosynthesis/degradation	Peptide (residues 421–428): IQSSLGGK	0.71#	0.25**	1.34*	0.68	0.53**	0.37**

One-way ANOVA, *p < 0.05 versus control group, **p < 0.01 versus control group. The value greater than 1 indicates up-regulation, less than 1 indicates down-regulation.

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