Figure 5
Biological application of the MyoPulser. (A) Shows the amount of protein carbonyls induced in cells by pulsing the sample for 6 h (biphasic pulses of 6 ms, gaps of 1 s). “Con” indicates a control kept for 6 h in the incubator under growth conditions (black column), the second control (“unpulsed”) was sham exposed (electrodes contacted the medium, but no pulsing was applied) besides of the “pulsed” sample (grey), while “pulsed” represents the sample actually electrically stimulated (white). No statistically significant differences were found between the samples (neither via ANOVA (Holm-Šídák) nor Student’s t-test, one-tailed, no correction, p < 0.05). (B) Compares the settings applied to the MyoPulser and the actual contraction times of pulsed cells, quantified using the software MYOCYTER. The grey columns indicate the sum of pulse and gap lengths actually applied using the MyoPulser, compared to the according averaged times between two subsequent contractions of the pulsed sample cells (white columns) in ms. “Average deviation from applied settings” (table below the panel) indicates the difference between applied settings and measured average times between two subsequent contractions in percent. “Standard deviation of measured time” indicates deviation from the actual measured cellular averages in percent (for example, 23 ms are 2.27% of 1012 ms).
