Figure 4 | Scientific Reports

Figure 4

From: Sortilin knock-down alters the expression and distribution of cathepsin D and prosaposin and up-regulates the cation-dependent mannose-6-phosphate receptor in rat epididymal cells

Figure 4

Expression and distribution of CD-MPR in RCE-1 and RCE-1 KD cells. (A) Representative immunoblot of CD-MPR in RCE-1 M (mock-depleted), RCE-1 and RCE-1 KD cells with their band intensity quantification. Bars represent the means of relative optical density (R.O.D.) ± SEM from three independent experiments. (***) significant difference from RCE-1 M and RCE-1 (p < 0.001). Ponceau S staining were used as loading control. The full-lenght immunoblot is presented in Supplementary Fig. 4. (B) Representative immunofluorescence staining of CD-MPR and CatD in RCE-1 and RCE-1 KD cells. Quantification of co-localization of CDMPR and CatD (MCC-M1); CatD and CD-MPR (MCC-M2). Values are expressed as the means of Manders colocalization coefficients 1 and 2 (MCC-M1 and MCC-M2, respectively) ± SEM. (***) significant difference from RCE-1 cells (p < 0.01). Scale bars = 10 μm.

Back to article page