Figure 6
Cdk5 enriched phosphoproteomics of rTBI. (A) Experimental design schematic displaying tissue isolation, extraction, Cdk5 motif enrichment, and peptide detection and analysis. (B) Volcano plot of differentially phosphorylated proteins between rTBI and con rats. (n = 5 pooled brains in duplicate per group) (increased sites in green decreased sites in red). (C) Volcano plot of differentially phosphorylated proteins between rTBI + 25–106 and rTBI alone rats. (D) Volcano plot of differentially phosphorylated proteins between rTBI + 25–106 and con rats. (E) Heatmap of all differentially phosphorylated sites across three comparisons in B-D expressed by fold change of each phosphor-site. (F) Venn diagram representing overlapping sites with positive FC ≥ 1.3 in rTBI:CON and negative FC in rTBI + 25–106:rTBI. (G) Dot-plot display of Ingenuity Pathway Analysis of phosphoproteins and canonical pathways upregulated FC ≥ 1.3 by rTBI:Con (Top) and downregulated with a FC ≤ − 1.3 by rTBI:Con (bottom). (H) Dot-plot display of Ingenuity Pathway Analysis of phosphoproteins and canonical pathways upregulated FC ≥ 1.3 by rTBI + 25,106:rTBI (Top) and downregulated with a FC ≤ − 1.3 by rTBI + 25–106:rTBI (bottom).
