Figure 2 | Scientific Reports

Figure 2

From: Flavescence dorée phytoplasma enters insect cells by a clathrin-mediated endocytosis allowing infection of its insect vector

Figure 2

Observation of Euva-12 and S2 cells 3 days post-infection with FD phytoplasmas (FDP). (a) Euva-12 infected with FDP. Each picture represents the maximum projection image of 5 z-planes. (b, c), S2 cells infected with FDP showing an external phytoplasma (b), and internalized phytoplasmas (c) in which the pictures represent the maximum projection image of 2 z-planes. The nuclei were stained with DAPI and coloured in magenta; the actin filaments were stained with Alexa 568-phalloidin and coloured in grey; external phytoplasmas were stained with Alexa 633 and coloured in red; after permeabilization of the infected cells, both internalized and external phytoplasmas were stained with Alexa 488 and coloured in green. Arrowheads indicate internalized phytoplasmas and arrows external phytoplasmas.

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