Figure 5

Observation of S2 cells expressing clathrin light chain tagged with GFP (GFP-Clc) and infected with FD phytoplasmas. (a, b) S2 cell expressing GFP-Clc and imaged at the surface of the cell (a) and inside the cell (b). Arrows indicate dots of GFP-Clc at the surface of the cells and arrowheads internal GFP-Clc dots. The nuclei were stained with DAPI and coloured in magenta; the GFP-Clc was coloured in green. (c, d), two different S2 cells expressing GFP-Clc and infected with FD phytoplasmas. The GFP-Clc was coloured in green, phytoplasmas were stained with Alexa 568 and coloured in red.