Table 3 Sequence of primers used in this study.

From: Flavescence dorée phytoplasma enters insect cells by a clathrin-mediated endocytosis allowing infection of its insect vector

Aplication	Primer name	5′–3′ sequence	Target gene	Species target/plasmid	Product size (n)	PCR efficiency (%)
dsRNA synthesis	S2-ClatHC-F3-T7	TAATACGACTCACTATAGGGGATGCGcGAACActTGGAgC	Clathrin heavy chain (chc)	Drosophila melanogaster	421	–
	S2-ClatHC-R3-T7	TAATACGACTCACTATAGGGGGTTATTGAGAGATTGGACTG	Clathrin heavy chain (chc)	Drosophila melanogaster	421	–
	Ev-ClatHCH1-T7-F2	TAATACGACTCACTATAGGGGTGTTGTTGAGGGACTGGACA	Clathrin heavy chain	Euscelidius variegatus	429	–
	Ev-ClatHCH1-T7-R2	TAATACGACTCACTATAGGGGTGCGAGAACATCTGGAACTG	Clathrin heavy chain	Euscelidius variegatus	429	–
	eGFP-ds-T7F1	TAATACGACTCACTATAGGGGCAAGGAGGACGGCAACATCC	GFP	pAGW	323	–
	eGFP-ds-T7R1	TAATACGACTCACTATAGGGGCGAACTCCAGCAGGACCATG	GFP	pAGW	323	–
Real-time PCR	S2-GST-F2	AAGTTGGTCACCCTGAATGC	Glutathione S-transferase (GST)	Drosophila melanogaster	180	107
	S2-GST-R2	TGGGTAGGGTTCCAACAG	Glutathione S-transferase (GST)	Drosophila melanogaster	180	107
	S2-EF1-F2	AGGCAGGTATCTCGAAGAAC	Elongation factor 1 α1 (EF1)	Drosophila melanogaster	103	107
	S2-EF1-R2	GAGGAGTCCATCTTGTTCAC	Elongation factor 1 α1 (EF1)	Drosophila melanogaster	103	107
	S2-ClatHC-F5	CATGCCGATGAGCTAGAGGA	Clathrin heavy chain	Drosophila melanogaster	128	100
	S2-ClatHC-R4	GCTAATTCGGTGAACATTCCC	Clathrin heavy chain	Drosophila melanogaster	128	100
	GST1_F257c^a	CCAAGGACCCCAAGAAGCGA	Glutathione S-transferase	Euscelidius variegatus	113	96
	GST1_R369c^a	TGGCGCTCCTCCAAACATCA	Glutathione S-transferase	Euscelidius variegatus	113	96
	EvTubB_F1	CGCCCAGAGGTCTCAAGATG	Tubuline β	Euscelidius variegatus	148	94
	EvTubB_R2	ATCTCGTCCATGCCCTCGC	Tubuline β	Euscelidius variegatus	148	94
	EvClatH1_F3	AGGCCACACATTTGAGCAAG	Clathrin heavy chain	Euscelidius variegatus	120	97
	EvClatH1_R1	GAAAGAGTTCCAGGGTGCAG	Clathrin heavy chain	Euscelidius variegatus	120	97
	3Fbl^b	TGAAGATCCAGTACGTGATTTAGAC	Elongation factor Tu (EFTu)	FD92 phytoplasma	158	96
	3R1^b	TTTTAGTTTCTTTAATACCTATGATTTC	Elongation factor Tu (EFTu)	FD92 phytoplasma	158	96
Cloning	GFP_clc_attB1-F	GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGACTTCGGAGACGATTTCG	Clathrin light chain	Drosophila melanogaster	745	–
	GFP_clc_attB2-R	GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAGGCGAGTGCGTAATTAAAACTAC	Clathrin light chain	Drosophila melanogaster	745	–
	pAG-bsd-F	GCAATTGTTGTTGTTAACTTGTTCCGAAGGGTTGTGTCAC	Blasticidin-S deaminase (bsd)	pIB/V5-His	650	–
	pAG-bsd-R	TGCAATAAACAAGTTAACCGGGACGTGTCAGTCCTGCT	Blasticidin-S deaminase (bsd)	pIB/V5-His	650	–

T7 promoter sequence is underlined.
^aPrimers were described in Galetto et al. (Galetto, L., Abbà, S., Rossi, M., Vallino, M., Pesando, M., Arricau-Bouvery, N., et al. (2018) Infect Immun 86: e00042–18).
^bPrimers were described in Eveillard et al. (Eveillard, S., Jollard, C., Labroussaa, F., Khalil, D., Perrin, M., Desqué, D., et al. (2016) Plant Biot Interact 1762).

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Table 3 Sequence of primers used in this study.

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