Table 4 Effect of the experimental diets and heat stress on the hematological counts of Nile tilapia (Oreochromis niloticus).
Treatment Indices | 25 °C | 40 °C | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CON | CN50 | CN100 | C-N200 | C50 | C100 | C200 | SDM | CON | CN50 | CN100 | C-N200 | C50 | C100 | C200 | SDM | |
Leukocytes | 34.99 | 32.77 | 36.51 | 32.41 | 38.02 | 38.22 | 50.56 | 6.45 | 49.86 | 53.01 | 57.44 | 53.39 | 54.11 | 61.05 | 80.39 | 11.17 |
Erythrocytes | 2.21 | 2.00 | 1.92 | 1.86 | 2.04 | 2.05 | 1.98 | 0.15 | 1.86 | 2.03 | 1.56 | 1.82 | 1.91 | 1.89 | 1.86 | 0.25 |
HGB | 5.90 | 5.70 | 5.25 | 5.70 | 5.40 | 5.65 | 5.95 | 0.50 | 5.40 | 6.55 | 4.50 | 6.30 | 5.45 | 6.55 | 5.75 | 0.83 |
HCT | 29.10 | 31.75 | 32.25 | 31.20 | 30.50 | 29.95 | 28.25 | 2.70 | 31.80 | 33.45 | 26.50 | 31.65 | 28.95 | 32.55 | 30.15 | 3.79 |
MCV | 131.60 | 158.30 | 167.55 | 169.05 | 150.15 | 146.00 | 142.70 | 17.24 | 171.75 | 165.50 | 168.00 | 172.30 | 153.35 | 172.50 | 162.20 | 11.97 |
MCH | 26.65 | 28.35 | 27.40 | 30.65 | 26.60 | 27.60 | 30.00 | 2.27 | 29.00 | 32.70 | 28.95 | 34.55 | 28.50 | 34.75 | 30.95 | 3.38 |
MCHC | 20.30 | 17.90 | 16.45 | 18.20 | 17.70 | 18.90 | 21.15 | 1.87 | 16.95 | 19.70 | 17.30 | 20.05 | 18.95 | 20.10 | 19.10 | 1.93 |
PLT | 21.00 | 25.00 | 23.50 | 23.00 | 25.00 | 24.00 | 24.50 | 2.16 | 51.50 | 51.50 | 66.50 | 58.00 | 53.00 | 57.00 | 57.00 | 4.94 |
NEUT | 5.70 | 5.30 | 5.85 | 5.30 | 5.90 | 6.75 | 15.05 | 3.53 | 17.60 | 16.90 | 17.55 | 16.55 | 17.15 | 20.40 | 36.15 | 7.11 |
LYMPH | 83.25 | 84.10 | 83.75 | 82.10 | 85.15 | 81.90 | 74.30 | 3.64 | 71.45 | 71.50 | 71.60 | 73.80 | 71.45 | 66.76 | 49.35 | 8.32 |
MONO | 1.80 | 1.10 | 2.45 | 2.15 | 1.25 | 0.80 | 0.35 | 0.99 | 1.00 | 0.40 | 1.25 | 0.55 | 1.15 | 1.05 | 0.75 | 0.37 |
EO | 0.05 | 0.10 | 0.10 | 0.25 | 0.15 | 0.05 | 0.45 | 0.16 | 0.15 | 0.20 | 0.10 | 0.05 | 0.70 | 0.85 | 1.65 | 0.76 |
BASO | 9.15 | 9.30 | 7.75 | 10.05 | 7.40 | 10.30 | 9.75 | 1.31 | 9.65 | 10.80 | 9.25 | 8.95 | 9.55 | 10.70 | 11.70 | 1.32 |
IG | 0.05 | 0.10 | 0.10 | 0.15 | 0.15 | 0.15 | 0.10 | 0.086 | 0.15 | 0.20 | 0.25 | 0.10 | 0.00 | 0.25 | 0.40 | 0.17 |
Variation source | Heat stress | Diet | Interaction | Duncan HSD for diets | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CON | CN50 | CN100 | CN200 | C50 | C100 | C200 | ||||||||||
Two-way ANOVA | ||||||||||||||||
Leukocytes | ⁎⁎⁎ | ⁎⁎ | * | b | b | b | b | b | b | a | ||||||
Erythrocytes | ns | ns | ns | a | a | a | a | a | a | a | ||||||
HGB | ns | ns | ns | ab | a | b | a | ab | a | a | ||||||
HCT | ns | ns | ns | a | a | a | a | a | a | a | ||||||
MCV | ⁎ | ns | ns | a | a | a | a | a | a | a | ||||||
MCH | ** | ns | ns | b | ab | b | a | b | ab | ab | ||||||
MCHC | ns | ns | ns | ab | ab | b | ab | ab | ab | a | ||||||
PLT | *** | ns | ns | a | a | a | a | a | a | a | ||||||
NEUT | *** | ⁎⁎⁎ | ns | b | b | b | b | b | b | a | ||||||
LYMPH | *** | ⁎⁎⁎ | ** | a | a | a | a | a | b | c | ||||||
MONO | ns | ns | ns | ab | ab | a | ab | ab | ab | b | ||||||
EO | ns | ns | ns | b | b | b | b | ab | ab | a | ||||||
BASO | * | ns | ns | ab | ab | b | ab | b | a | a | ||||||
IG | ns | ns | ns | a | a | a | a | a | a | a | ||||||
