Figure 10
From: Recombinant anti-HIV MAP30, a ribosome inactivating protein: against plant virus and bacteriophage

The inoculation of C. quinoa seedlings simultaneously with purified CMV and rMAP30 or non-transgenic protein. The extraction buffer (phosphate buffer) was considered as one of the negative controls (Table 4). There were 16 replicates for each test. (a) The assays performed by undiluted rMAP30 (T), extraction buffer (B), non-transgenic protein (N), and CMV (C). (b) Viral infection intensity graph for non-diluted transgenic rMAP30 versus non-transgenic protein and extraction buffer. As it could be observed in the figures and the chart, rMAP30 can control the infection up to 74% in the undiluted stats. Although the extraction buffer, non-transgenic protein, as the control samples, were implemented in order to achieve a better understanding, that they did not influence the viral infection reduction. In addition, it was found that the intensity of viral spots on the controls inoculated leaves was highly similar to the virus-inoculated leaves.