Figure 2

Formation and elongation of N. tobacco hairy roots at different periods after the A. rhizogenes infection. (a) The emergence of transgenic hairy roots from bacterial inoculated leaf explants grown on a solid MS medium with 30 mg/L of meropenem and 100 mg/L of kanamycin, which had been maintained at 25 °C under a 16/8 h light/dark photoperiod for 2 weeks after the infection; (b) the growth pattern of non-transgenic hairy root samples on kanamycin, and non-kanamycin culture media. The inability to grow on a medium that contains kanamycin and meropenem, and the growth process on an environment that only contains meropenem indicates that they are not transgenic; therefore, they have not received the expression construct (Mer indicates meropenem antibiotic, and Kan indicates kanamycin antibiotic). (c) The growth of hairy roots cultivated in a 250-mL Erlenmeyer flask that contains MS liquid medium, which is refreshed weekly and only contains meropenem antibiotic at 28 °C in the darkness with gentle shaking for 1 or 2 months. (d) Confirmation of the production of rMAP30 in the hairy roots of tobacco plant tissues using ELISA, BSA indicates bovine serum albumin.