Table 1 Sequences of the primers applied for PCR-based characterization of transgenic hairy roots. Moghadam et al., 2016 designed primers using Vector NTI 11 and Allele ID 7 software19.
From: Recombinant anti-HIV MAP30, a ribosome inactivating protein: against plant virus and bacteriophage
Primer name | Sequence | Ta (°C) | Product length (bp) |
|---|---|---|---|
MAP30-F | ATGGCACCACAAAAGGAGAAC | 58 | 861 |
MAP30-R | AACCTGAAACCTTTCTCCTGTAG | ||
RolB-F | AAGTGCTGAAGGAACAATC | 54 | 194 |
RolB-R | CAAGTGAATGAACAAGGAAC | ||
VirG-F | CCTTGGGCGTCGTCATAC | 55 | 529 |
VirG-F | TCGTCCTCGGTCGTTTCC |
