Table 4 Anti-CMV activity of MAP30.

From: Recombinant anti-HIV MAP30, a ribosome inactivating protein: against plant virus and bacteriophage

Simultaneous assay

Protein

Volume (μg)

CMV

Volume (μg)

Undiluted rMAP30

0.6

Purified

0.1 (μg)

Undiluted rMAP30

0.6

Extraction-infected virusa

15 (μL)

1/2 diluted rMAP30

0.3

Extraction-infected virus

15 (μL)

1/4 diluted rMAP30

0.15

Extraction-infected virus

15 (μL)

Time interval assay

Protein

Volume (μg)

CMV

Volume (μg)

rMAP30

1

Purified

0.1

  1. The different concentrations of purified recombinant MAP30 and the total protein extracted from transgenic lines are shown. Simultaneous assay indicates the treatments using MAP30 and CMV at the same time. Time interval assay indicates the treatments using MAP30 and CMV separately and in a period of the time.
  2. aThis means the total extraction was obtained from virus infected leaves of the host.
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