Figure 1 | Scientific Reports

Figure 1

From: Prostate cancer cell-platelet bidirectional signaling promotes calcium mobilization, invasion and apoptotic resistance via distinct receptor-ligand pairs

Figure 1

PCa cells physically interact with platelets. Representative fluorescent images of (A) RC77 T/E and (B) MDA PCa 2b cells incubated with platelets at a cell:platelet ratio = 1:500. Cells were stained with antibody for the P2Y12 receptor (cyan) and DAPI for DNA (blue). Platelets were stained with an antibody for CD41a (red). Inset depicts scale bar in 5 µm intervals. (C) Quantification of platelet singlets and clusters/aggregates interacting with RC77 T/E (n = 109 imaged cells incubated with platelets at a cell:platelet ratio = 1:500) and MDA PCa 2b (n = 127 imaged cells incubated with platelets at a cell:platelet ratio = 1:500). Bar graphs represent the mean ± SEM; Student’s t-test, *P < 0.05. (D) Representative transmission electron micrograph (TEM) images depicting physical interactions between RC77 T/E cells and platelets. (E) Magnified image of microvilli-like structures projecting from RC77 T/E cell toward platelet aggregates (top panel) and representative image of platelet-platelet focal adhesions indicative of PCa cell-stimulated platelet aggregation (bottom panel).

Back to article page